Primary structure of the putative human oncogene, pim-1.
Gene
; 90(2): 303-7, 1990 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-2205533
ABSTRACT
A full-length (6.1-kb) human genomic pim-1 gene, together with its immediate 5'-upstream promoter sequence (Ppim) was isolated and sequenced. The human pim-1 gene shares an overall nucleotide (nt) sequence identity of 53% with the previously reported murine pim-1 gene. It consists of six exons and five introns and contains a protein-coding region that is identical in nt sequence to a full-length human pim-1 cDNA. The gene codes for a predicted Pim-1 protein of 313 amino acids (aa) with an Mr of 35,690 and a pI of 5.7. The deduced aa sequence of the human Pim-1 has 94% identity with the murine Pim-1 whereas the nt sequences of the two genes are 88% identical. All of the conserved aa residues of the mouse pim-1 gene, which are homologous to known protein kinases are conserved in the predicted human protein. The human Ppim region is very G + C-rich (69%) and shares greater than 80% identity with the murine Ppim. The Ppim has no TATA- or CAAT-box sequences but does contain a number of nt sequences similar to the putative binding sites of several presumptive transcription factors.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oncogenes
/
Proto-Oncogene Proteins
/
Protein Serine-Threonine Kinases
Type of study:
Prognostic_studies
/
Screening_studies
Aspects:
Patient_preference
Limits:
Animals
/
Humans
Language:
En
Journal:
Gene
Year:
1990
Document type:
Article