The quantitative proteome of a human cell line.
Mol Syst Biol
; 7: 549, 2011 Nov 08.
Article
in En
| MEDLINE
| ID: mdl-22068332
ABSTRACT
The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least -10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteome
/
Gene Expression Profiling
Limits:
Humans
Language:
En
Journal:
Mol Syst Biol
Journal subject:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
Germany