Engineering the cytokinin-glucoside specificity of the maize ß-D-glucosidase Zm-p60.1 using site-directed random mutagenesis.

Filipi, Tomás; Mazura, Pavel; Janda, Lubomír; Kiran, Nagavalli S; Brzobohatý, Bretislav

Filipi, Tomás; Mazura, Pavel; Janda, Lubomír; Kiran, Nagavalli S; Brzobohatý, Bretislav.

Affiliation

Filipi T; Laboratory of Plant Molecular Biology, Institute of Biophysics AS CR, vvi and Mendel University in Brno, Zemedelská 1, CZ-613 00 Brno, Czech Republic.

Phytochemistry ; 74: 40-8, 2012 Feb.

Article in En | MEDLINE | ID: mdl-22079107

Subject(s)

Amino Acids/metabolism; Cytokinins/metabolism; Glucosides/metabolism; Plant Proteins/chemistry; Zea mays/enzymology; beta-Glucosidase/chemistry; Amino Acid Sequence; Binding Sites; Genes, Plant; Hydrolysis; Isomerism; Molecular Conformation; Mutagenesis, Site-Directed/methods; Mutation; Plant Proteins/genetics; Structure-Activity Relationship; Substrate Specificity; Zea mays/chemistry; Zea mays/genetics; Zeatin/metabolism; beta-Glucosidase/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Beta-Glucosidase / Zea mays / Cytokinins / Amino Acids / Glucosides Type of study: Clinical_trials Language: En Journal: Phytochemistry Year: 2012 Document type: Article Affiliation country: Czech Republic Country of publication: United kingdom

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