The 5' leader sequence of mouse mammary tumor virus enhances expression of the envelope and reporter genes.
J Gen Virol
; 93(Pt 2): 308-318, 2012 Feb.
Article
in En
| MEDLINE
| ID: mdl-22113011
ABSTRACT
Mouse mammary tumor virus (MMTV) is a complex betaretrovirus, which utilizes a Rev-like auxiliary protein Rem to export the unspliced viral RNA from the nucleus. MMTV env mRNA appears to be exported via a distinct, Rem-independent, mechanism. Here, we analysed the effect of an extensively folded region coinciding with the 5' leader sequence on env gene expression. We found that the presence of the 5' leader stimulates expression of the envelope protein. Enhanced Env production was accompanied by increased cytoplasmic levels of env mRNA. The 5' leader promotes nucleocytoplasmic translocation and increases stability of env mRNA. The region responsible for this effect was mapped to the distal part of the 5' leader. Furthermore, the 5' leader inserted in the sense orientation into a heterologous luciferase expression construct increased luciferase activity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virus Replication
/
Viral Envelope Proteins
/
Mammary Tumor Virus, Mouse
/
5' Untranslated Regions
Limits:
Animals
/
Humans
Language:
En
Journal:
J Gen Virol
Year:
2012
Document type:
Article
Affiliation country:
Austria