Fludarabine nucleoside modulates nuclear "survival and death" proteins in resistant chronic lymphocytic leukemia cells.
Nucleosides Nucleotides Nucleic Acids
; 30(12): 1181-9, 2011 Dec.
Article
in En
| MEDLINE
| ID: mdl-22132973
ABSTRACT
The nuclear mechanisms by which fludarabine nucleoside (F-ara-A) induces apoptosis have been investigated in human MEC1 cells derived from B-cell chronic lymphocytic leukemia. Upon treatment of cells with F-ara-A (100 µM, 72 hours), 15 nuclear proteins changed in abundance by more than 2-fold. Nuclear proteins up-regulated included calmodulin (4.3-fold), prohibitin (3.9-fold), ß-actin variant (3.7-fold), and structure-specific recognition protein 1 (3.7-fold); those down-regulated included 60S ribosomal protein P2B (0.12-fold), fumarate hydratase (0.19-fold), splicing factor arginine/serine-rich 3 (0.35-fold), and replication protein A2 (0.42-fold). These changes in the levels of specific proteins promote survival or apoptosis; because the end result is apoptosis of MEC1 cells, apoptotic effects predominate.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vidarabine
/
Nuclear Proteins
/
Leukemia, Lymphocytic, Chronic, B-Cell
/
Drug Resistance, Neoplasm
/
Nucleosides
Limits:
Humans
Language:
En
Journal:
Nucleosides Nucleotides Nucleic Acids
Journal subject:
BIOQUIMICA
Year:
2011
Document type:
Article
Affiliation country:
Australia