L-arginine is a radioprotector for hematopoietic progenitor cells.
Radiat Res
; 177(6): 792-803, 2012 Jun.
Article
in En
| MEDLINE
| ID: mdl-22175298
ABSTRACT
L-arginine is shown to protect hematopoietic progenitor (32D cl 3) cells from death due to exposure to γ radiation ((137)Cs). Some of the other intermediates in the urea cycle, namely ornithine and citrulline, plus urea itself, were not found to have any significant impact on cell survival after irradiation. Intriguingly, supplementation of irradiated cells with L-arginine results in decreased production of peroxynitrite, suggesting that suppression of superoxide generation by nitric oxide synthase in one or more microenvironments is an important factor in the observed radioprotection. The absence of any radioprotective effect of L-arginine in cells at 3% oxygen also confirms the involvement of one or more oxygen-derived species. Knockdown experiments with nitric oxide synthase (NOS) siRNAs in cells and NOS knockout animals confirm that the observed radioprotection is associated with nNOS (NOS-1). L-arginine also ameliorates the transient inhibition of the electron-transport chain complex I that occurs within 30 min of completing the dose (10 Gy) and that appears to be a functional marker for postirradiation mitochondrial oxidant production.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arginine
/
Radiation-Protective Agents
/
Hematopoietic Stem Cells
Limits:
Animals
Language:
En
Journal:
Radiat Res
Year:
2012
Document type:
Article
Affiliation country:
United States