MAPPIT as a high-throughput screening assay for modulators of protein-protein interactions in HIV and HCV.
Methods Mol Biol
; 812: 295-307, 2012.
Article
in En
| MEDLINE
| ID: mdl-22218868
ABSTRACT
The discovery of novel antivirals for HIV and HCV has been a focus of intensive research for many years. Where the inhibition of critical viral enzymes by small molecules has proven effective for many viruses, there is considerable merit in pursuing protein-protein interactions (PPIs) as targets for therapeutic intervention. The mammalian protein-protein interaction trap (MAPPIT) is a two-hybrid system used for the study of PPIs. The bait and prey proteins are linked to deficient cytokine receptor chimeras, where the bait and prey interaction and subsequent ligand stimulation restores JAK-STAT signaling, resulting in reporter gene expression controlled by a STAT3-responsive promoter. We report the use of MAPPIT as a high-throughput screening assay for the discovery of inhibitors or stimulators of the Vif-APOBEC3G interaction and the reverse transcriptase heterodimerization (RTp66-RTp51) for HIV and the NS4A-NS3 interaction for HCV.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
HIV
/
Hepacivirus
/
Drug Evaluation, Preclinical
/
High-Throughput Screening Assays
/
Protein Interaction Maps
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2012
Document type:
Article
Affiliation country:
Belgium