Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: molecular insights and effector modulation of N-acetylglucosaminyltransferase I.

Zhong, Xiaotian; Cooley, Cecilia; Seth, Nilufer; Juo, Z Sean; Presman, Ella; Resendes, Nicole; Kumar, Ravi; Allen, Martin; Mosyak, Lidia; Stahl, Mark; Somers, Will; Kriz, Ronald

Zhong, Xiaotian; Cooley, Cecilia; Seth, Nilufer; Juo, Z Sean; Presman, Ella; Resendes, Nicole; Kumar, Ravi; Allen, Martin; Mosyak, Lidia; Stahl, Mark; Somers, Will; Kriz, Ronald.

Affiliation

Zhong X; Global BioTherapeutics Technologies, Pfizer BioTherapeutic Research and Development, 87 Cambridge Park Drive, Cambridge, Massachusetts 02140, USA. xiaotian.zhong@pfizer.com

Biotechnol Bioeng ; 109(7): 1723-34, 2012 Jul.

Article in En | MEDLINE | ID: mdl-22252477

Subject(s)

Mutation; N-Acetylglucosaminyltransferases/genetics; Oligosaccharides/metabolism; Protein Engineering/methods; Amino Acid Sequence; Animals; CHO Cells; Cell Line; Clone Cells; Cricetinae; Glycosylation; Humans; Immunoglobulin G/genetics; Immunoglobulin G/metabolism; Models, Molecular; Molecular Sequence Data; N-Acetylglucosaminyltransferases/chemistry; N-Acetylglucosaminyltransferases/metabolism; Oligosaccharides/genetics; Rabbits; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Alignment

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligosaccharides / Protein Engineering / N-Acetylglucosaminyltransferases / Mutation Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Biotechnol Bioeng Year: 2012 Document type: Article Affiliation country: United States Country of publication: United States

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