No correlation between pinopode formation and LIF and MMP2 expression in endometrium during implantation window.
Folia Histochem Cytobiol
; 49(4): 615-21, 2011.
Article
in En
| MEDLINE
| ID: mdl-22252755
ABSTRACT
Implantation depends on two factors - embryo and endometrium. The period of maximal endometrial receptivity is a poorly understood phenomenon. We decided to look at three possible markers of implantation pinopodes, leukemia inhibitory factor, and matrix metalloproteinase 2 and their correlations. We included in the study 23 idiopathic infertility patients and 21 patients with recurrent spontaneous abortions of unknown etiology. Twenty one fertile patients were also recruited. A biopsy was used for endometrial dating according to the Noyes and Hertig criteria, and assessed for the presence of pinopodes via a scanning electron microscope. Endometria were examined in Real Time-Polymerase Chain Reaction cycles for the mRNA expression of leukemia inhibitory factor (LIF) and matrix metalloproteinase 2 (MMP2). No difference was found in the stage of pinopodes development, nor in the coverage of endometrial surface between the studied groups. The expression level for LIF mRNA was lower in control patients compared to idiopathic infertility and recurrent miscarriage patients. No difference was detected in the expression of MMP2 between all studied groups. No correlation was found between pinopodes development stage and LIF and MMP2 expressions in endometrium. Of the studied factors, LIF and pinopodes show the most promise as potential markers of endometrial receptivity. However, the results achieved suggest that these markers are independent of each other.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Embryo Implantation
/
Matrix Metalloproteinase 2
/
Endometrium
/
Leukemia Inhibitory Factor
/
Infertility, Female
Limits:
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Folia Histochem Cytobiol
Journal subject:
HISTOCITOQUIMICA
Year:
2011
Document type:
Article
Affiliation country:
Poland