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In vitro platelet quality in storage containers used for pediatric transfusions.
Weiss, Sandra; Scammell, Kenneth; Levin, Elena; Culibrk, Brankica; Zolfaghari, Sima; Gyöngyössy-Issa, Maria I C; Acker, Jason P.
Affiliation
  • Weiss S; Canadian Blood Services Research and Development, Edmonton, Alberta, Canada.
Transfusion ; 52(8): 1703-14, 2012 Aug.
Article in En | MEDLINE | ID: mdl-22257224
BACKGROUND: The in vitro quality of small-volume platelet (PLT) aliquots for pediatric transfusions was assessed to determine the best practice approach. STUDY DESIGN AND METHODS: Small volumes (50 mL) of single apheresis PLT components (APCs), collected on either CaridianBCT Trima or Haemonetics MCS+ instruments, were aliquoted on Days 2, 3, 4, and 5 postcollection into Fenwal PL1240 or 4R2014 bags or 60-mL polypropylene syringes. Samples were tested for in vitro quality at their recommended expiry times (4 hr for 4R2014 bags and syringes or Day 5 for PL1240 bags). Assays included pH, CD62P expression, and metabolic measures. RESULTS: CD62P expression increased throughout storage in all containers. Among the small-volume containers, pH, pCO(2) , lactate, and bicarbonate varied considerably. Regardless of the day of aliquoting, pCO(2) was significantly higher and pO(2) was significantly lower in gas-impermeable syringes than other containers. No bacterial growth was detected in any sample. CONCLUSION: The quality of APCs aliquoted into small-volume containers meets regulatory requirements and is generally equivalent to that of full-volume APCs at expiry.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Banks / Blood Platelets / Blood Preservation / Platelet Transfusion Type of study: Guideline Limits: Child / Humans Language: En Journal: Transfusion Year: 2012 Document type: Article Affiliation country: Canada Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood Banks / Blood Platelets / Blood Preservation / Platelet Transfusion Type of study: Guideline Limits: Child / Humans Language: En Journal: Transfusion Year: 2012 Document type: Article Affiliation country: Canada Country of publication: United States