ORF23 of murine gammaherpesvirus 68 is non-essential for in vitro and in vivo infection.
J Gen Virol
; 93(Pt 5): 1076-1080, 2012 May.
Article
in En
| MEDLINE
| ID: mdl-22258865
ABSTRACT
Although ORF23 is conserved among gammaherpesviruses, its role during infection is unknown. Here, we studied the expression of ORF23 of murine gammaherpesvirus 68 (MHV-68) and its role during infection. ORF23 mRNA was detected in infected cells as a late transcript. The ORF23 protein product could be expressed and detected as an N-terminally FLAG-tagged protein by Western blot and indirect immunofluorescence. To investigate the role of ORF23 in the infection cycle of a gammaherpesvirus, we constructed an ORF23 deletion mutant of MHV-68. The analysis of the ORF23 deletion mutant suggested that ORF23 of MHV-68 is neither essential for replication in cell culture nor for lytic or latent infection in vivo. A phenotype of the ORF23 deletion mutant, reflected by a moderate reduction in lytic replication and latency amplification, was only detectable in the face of direct competition to the parental virus.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Proteins
/
Virus Replication
/
Open Reading Frames
/
Rhadinovirus
Limits:
Animals
Language:
En
Journal:
J Gen Virol
Year:
2012
Document type:
Article
Affiliation country:
Germany