Analysis of the 5' untranslated region (5'UTR) of the alcohol oxidase 1 (AOX1) gene in recombinant protein expression in Pichia pastoris.

Staley, Chris A; Huang, Amy; Nattestad, Maria; Oshiro, Kristin T; Ray, Laura E; Mulye, Tejas; Li, Zhiguo Harry; Le, Thu; Stephens, Justin J; Gomez, Seth R; Moy, Allison D; Nguyen, Jackson C; Franz, Andreas H; Lin-Cereghino, Joan; Lin-Cereghino, Geoff P

Staley, Chris A; Huang, Amy; Nattestad, Maria; Oshiro, Kristin T; Ray, Laura E; Mulye, Tejas; Li, Zhiguo Harry; Le, Thu; Stephens, Justin J; Gomez, Seth R; Moy, Allison D; Nguyen, Jackson C; Franz, Andreas H; Lin-Cereghino, Joan; Lin-Cereghino, Geoff P.

Affiliation

Staley CA; Department of Biological Sciences, University of the Pacific, Stockton, CA 95211, USA.

Gene ; 496(2): 118-27, 2012 Apr 01.

Article in En | MEDLINE | ID: mdl-22285974

Subject(s)

5' Untranslated Regions; Alcohol Oxidoreductases/genetics; Gene Expression Regulation, Fungal; Pichia/metabolism; Base Sequence; Cell-Free System; Gene Deletion; Gene Expression Profiling/methods; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Nucleic Acid Conformation; Protein Biosynthesis; Real-Time Polymerase Chain Reaction/methods; Recombinant Proteins/metabolism; beta-Galactosidase/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pichia / Gene Expression Regulation, Fungal / 5' Untranslated Regions / Alcohol Oxidoreductases Language: En Journal: Gene Year: 2012 Document type: Article Affiliation country: United States Country of publication: Netherlands

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