Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis.
Anal Biochem
; 424(1): 54-6, 2012 May 01.
Article
in En
| MEDLINE
| ID: mdl-22342882
ABSTRACT
The H1N1 influenza virus has spread worldwide to become pandemic. Here, we developed a new method to discriminate various types of influenza A, including H1N1, using stuffer-free multiplex ligation-dependent probe amplification based on a conformation-sensitive separation method, namely capillary electrophoresis-single-strand conformation polymorphism. Unlike conventional methods, our approach precisely detects five relevant gene markers permitting confirmation of infection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine
/
DNA, Viral
/
Orthomyxoviridae Infections
/
Electrophoresis, Capillary
/
Influenza A Virus, H1N1 Subtype
/
Multiplex Polymerase Chain Reaction
/
Nucleic Acid Conformation
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Anal Biochem
Year:
2012
Document type:
Article