Brain microdialysis in freely moving animals.
Methods Mol Biol
; 846: 365-81, 2012.
Article
in En
| MEDLINE
| ID: mdl-22367826
ABSTRACT
Brain microdialysis is an analytical technique used for the dynamic monitoring of brain neurochemistry in awake, freely moving animals. This technique requires the insertion of a small dialysis catheter, called a microdialysis probe, into a specific brain region, and its perfusion with an artificial extracellular fluid. The microdialysate samples, obtained from the probe outlet, can be analysed using high-performance liquid chromatography with electrochemical detection for the quantification of oxidizable molecules recovered from the extracellular space. In this chapter, we describe a protocol for performing a microdialysis setup and experiment in freely moving rats and mice. Furthermore, the high-performance liquid chromatographic determination of ascorbic acid, uric acid, catecholamines, indolamines and derivatives is described in detail.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Chemistry
/
Chemistry Techniques, Analytical
/
Microdialysis
/
Extracellular Fluid
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2012
Document type:
Article
Affiliation country:
Italy