Dihydroarteminsin-induced apoptosis is not dependent on the translocation of Bim to the endoplasmic reticulum in human lung adenocarcinoma cells.
Pathol Oncol Res
; 18(4): 809-16, 2012 Oct.
Article
in En
| MEDLINE
| ID: mdl-22391963
ABSTRACT
Bim, a proapoptotic BH3-only member of Bcl-2 family, has been considered to play an important role in initiating mitochondrial apoptotic pathway. Our previous studies have shown the ability of dihydroarteminsin (DHA) to induce apoptosis in human lung adenocarcinoma (ASTC-a-1) cells. In this study, we investigated the function of Bim during DHA-induced apoptosis in ASTC-a-1 and another human lung adenocarcinoma (A549) cell lines. Confocal imaging of single living cell expressing GFP-BimL showed the translocation of Bim to endoplasmic reticulum (ER) rather than mitochondria during DHA-induced apoptosis. Moreover, we also found that DHA induced ER stress and an increase of Bim protein levels. However, silencing Bim by short hairpin RNA did not inhibit DHA-induced caspase-9 activation and cell apoptosis. Taken together, our results demonstrate for the first time that DHA induces Bim translocation to ER, but DHA-induced apoptosis is not dependent on Bim in ASTC-a-1 and A549 cell lines.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenocarcinoma
/
Proto-Oncogene Proteins
/
Apoptosis
/
Artemisinins
/
Endoplasmic Reticulum
/
Apoptosis Regulatory Proteins
/
Lung Neoplasms
/
Membrane Proteins
Limits:
Humans
Language:
En
Journal:
Pathol Oncol Res
Journal subject:
NEOPLASIAS
/
PATOLOGIA
Year:
2012
Document type:
Article
Affiliation country:
China