PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking.
Opt Express
; 20(5): 4957-67, 2012 Feb 27.
Article
in En
| MEDLINE
| ID: mdl-22418300
ABSTRACT
We present a novel approach for three-dimensional localization of single molecules using adaptive optics. A 52-actuator deformable mirror is used to both correct aberrations and induce two-dimensional astigmatism in the point-spread-function. The dependence of the z-localization precision on the degree of astigmatism is discussed. We achieve a z-localization precision of 40 nm for fluorescent proteins and 20 nm for fluorescent dyes, over an axial depth of ~800 nm. We illustrate the capabilities of our approach for three-dimensional high-resolution microscopy with super-resolution images of actin filaments in fixed cells and single-molecule tracking of quantum-dot labeled transmembrane proteins in live HeLa cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Image Enhancement
/
Proteins
/
Lenses
/
Microscopy
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Opt Express
Journal subject:
OFTALMOLOGIA
Year:
2012
Document type:
Article
Affiliation country:
France