An easy and versatile 2-step protocol for targeted modification and subcloning of DNA from bacterial artificial chromosomes using non-commercial plasmids.

Hartwich, Heiner; Nothwang, Hans Gerd

Hartwich, Heiner; Nothwang, Hans Gerd.

Affiliation

Hartwich H; Department of Neurogenetics, Institute of Biology and Environmental Sciences, Carl von Ossietzky University, Carl-von-Ossietzky-Strasse 9-11, 26129 Oldenburg, Germany. heiner.hartwich@uni-oldenburg.de

BMC Res Notes ; 5: 156, 2012 Mar 20.

Article in En | MEDLINE | ID: mdl-22433714

Subject(s)

Chromosomes, Artificial, Bacterial; Cloning, Molecular/methods; DNA/genetics; Escherichia coli/genetics; Plasmids; Genetic Engineering; Mutagenesis, Site-Directed; Recombination, Genetic

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasmids / DNA / Cloning, Molecular / Chromosomes, Artificial, Bacterial / Escherichia coli Language: En Journal: BMC Res Notes Year: 2012 Document type: Article Affiliation country: Germany Country of publication: United kingdom

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