An engineered eukaryotic protein glycosylation pathway in Escherichia coli.
Nat Chem Biol
; 8(5): 434-6, 2012 Mar 25.
Article
in En
| MEDLINE
| ID: mdl-22446837
ABSTRACT
We performed bottom-up engineering of a synthetic pathway in Escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. The glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, we successfully transferred glycans to eukaryotic proteins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
Protein Engineering
/
Escherichia coli Proteins
/
Disaccharides
/
Escherichia coli
Language:
En
Journal:
Nat Chem Biol
Journal subject:
BIOLOGIA
/
QUIMICA
Year:
2012
Document type:
Article
Affiliation country:
United States