Combined antibacterial activity of phage lytic proteins holin and lysin from Streptococcus suis bacteriophage SMP.
Curr Microbiol
; 65(1): 28-34, 2012 Jul.
Article
in En
| MEDLINE
| ID: mdl-22526567
ABSTRACT
Development of novel antibacterial agents is required to control infection with multidrug-resistant Streptococcus suis. HolSMP and LySMP, the holin and lysin of S. suis serotype 2 bacteriophage, named SMP, are responsible for lysis of host cells and release of progeny phage. HolSMP and LySMP expressed in Escherichia coli BL21(DE3) exerted efficient activity at 37 °C, pH 5.2, with addition of 0.8 % ß-mercaptoethanol. Lytic spectra of purified HolSMP, LySMP or HolSMP + LySMP mixture were investigated. HolSMP, exhibiting a narrow lytic spectrum, was effective against Staphylococcus aureus and Bacillus subtilis, which were insensitive to LySMP. Moreover, HolSMP was identified as a promising antibacterial agent which was able to extend the spectrum of LySMP. The data suggest that combined use of holin and lysin could be a candidate strategy for resolution of drug resistance.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Proteins
/
Streptococcus Phages
/
Enzymes
/
Anti-Bacterial Agents
Language:
En
Journal:
Curr Microbiol
Year:
2012
Document type:
Article