Inhibited experimental corneal neovascularization by neutralizing anti-SDF-1α antibody.
Int J Ophthalmol
; 5(1): 7-12, 2012.
Article
in En
| MEDLINE
| ID: mdl-22553746
ABSTRACT
AIM:
To explore the effect of SDF-1α on the development of experimental corneal neovascularization (CRNV).METHODS:
CRNV was induced by alkali injury in mice. The expression of SDF-1α and CXCR4 in burned corneas was examined by Flow Cytometry. Neutralizing anti-mouse SDF-1α antibody was locally administrated after alkali injury and the formation of CRNV 2 weeks after injury was assessed by Immunohistochemistry. The expression of VEGF and C-Kit in burned corneas was detected by RT-PCR.RESULTS:
The number of CRNV peaks at 2 weeks after alkali injury. Compared to control group, SDF-1α neutralizing antibody treatment significantly decreased the number of CRNV. RT-PCR confirmed that SDF-1α neutralizing antibody treatment resulted in decreased intracorneal VEGF and C-Kit expression.CONCLUSION:
SDF-1α neutralizing antibody treated mice exhibited impaired experimental CRNV through down regulated VEGF and C-Kit expression.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Int J Ophthalmol
Year:
2012
Document type:
Article
Affiliation country:
China