New phosphate binding sites in the crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with phosphate and formycin A.

Stefanic, Zoran; Narczyk, Marta; Mikleusevic, Goran; Wielgus-Kutrowska, Beata; Bzowska, Agnieszka; Luic, Marija

Stefanic, Zoran; Narczyk, Marta; Mikleusevic, Goran; Wielgus-Kutrowska, Beata; Bzowska, Agnieszka; Luic, Marija.

Affiliation

Stefanic Z; Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia.

FEBS Lett ; 586(7): 967-71, 2012 Apr 05.

Article in En | MEDLINE | ID: mdl-22569248

Subject(s)

Antineoplastic Agents/metabolism; Enzyme Inhibitors/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli/enzymology; Formycins/metabolism; Phosphates/metabolism; Purine-Nucleoside Phosphorylase/chemistry; Antineoplastic Agents/chemistry; Binding Sites; Crystallography, X-Ray; Enzyme Inhibitors/chemistry; Escherichia coli Proteins/antagonists & inhibitors; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Formycins/chemistry; Kinetics; Ligands; Models, Molecular; Osmolar Concentration; Phosphates/chemistry; Protein Conformation; Purine-Nucleoside Phosphorylase/antagonists & inhibitors; Purine-Nucleoside Phosphorylase/genetics; Purine-Nucleoside Phosphorylase/metabolism; Recombinant Proteins/antagonists & inhibitors; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Spectrometry, Fluorescence; Titrimetry; Water/chemistry; Water/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphates / Formycins / Purine-Nucleoside Phosphorylase / Escherichia coli Proteins / Enzyme Inhibitors / Escherichia coli / Antineoplastic Agents Language: En Journal: FEBS Lett Year: 2012 Document type: Article Affiliation country: Croatia

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