Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system.
Biotechnol Lett
; 34(9): 1623-7, 2012 Sep.
Article
in En
| MEDLINE
| ID: mdl-22576283
ABSTRACT
The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biotechnology
/
Gene Expression
/
Capsid Proteins
/
Antigens, Viral
Limits:
Animals
/
Humans
Language:
En
Journal:
Biotechnol Lett
Year:
2012
Document type:
Article
Affiliation country:
Brazil