An LC-MS/MS method for determination of jujuboside A in rat plasma and its application to pharmacokinetic studies.
J Chromatogr B Analyt Technol Biomed Life Sci
; 899: 21-6, 2012 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-22622062
ABSTRACT
A novel, simple, and sensitive method for the determination of jujuboside A in rat plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed. Following solid-phase extraction, measurement of jujuboside A was performed by negative ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The limit of detection was 1.25 ng/mL, and the lower limit of quantification was 5 ng/mL in rat plasma. Good linearity was obtained over the range of 6.25-500 ng/mL, and the correlation coefficient was better than 0.998. The intra- and inter-day precisions ranged 4.4-7.5% and 2.9-10.7%, respectively. The accuracy derived from QC samples ranged 3.2-7.8% and 2.2-3.5%, respectively. The recovery ranged from 72.9 to 75.1% and the matrix effect from 96.7 to 105.3%. The analyte was stable under various conditions (at room temperature, during freeze-thaw, in the autosampler and under deep-freeze conditions). The developed method was successfully applied to the pharmacokinetic study in rats.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saponins
/
Drugs, Chinese Herbal
/
Chromatography, High Pressure Liquid
/
Ziziphus
/
Tandem Mass Spectrometry
Type of study:
Evaluation_studies
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2012
Document type:
Article
Affiliation country:
China