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Prevalence and antimicrobial resistance of Enterococcus spp and Staphylococcus spp isolated from surfaces in a veterinary teaching hospital.
Hamilton, Elizabeth; Kaneene, John B; May, Katherine J; Kruger, John M; Schall, William; Beal, Matthew W; Hauptman, Joe G; DeCamp, Charles E.
Affiliation
  • Hamilton E; Center for Comparative Epidemiology, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
J Am Vet Med Assoc ; 240(12): 1463-73, 2012 Jun 15.
Article in En | MEDLINE | ID: mdl-22657930
ABSTRACT

OBJECTIVE:

To determine the prevalence and antimicrobial resistance of enterococci and staphylococci collected from environmental surfaces at a veterinary teaching hospital (VTH).

DESIGN:

Longitudinal study. SAMPLE Samples collected from surfaces in 5 areas (emergency and critical care, soft tissue and internal medicine, and orthopedic wards; surgery preparation and recovery rooms; and surgery office and operating rooms) of a VTH. PROCEDURES Selected surfaces were swabbed every 3 months during the 3-year study period (2007 to 2009). Isolates of enterococci and staphylococci were identified via biochemical tests, and antimicrobial susceptibility was evaluated with a microbroth dilution technique. A subset of isolates was analyzed to assess clonality by use of pulsed-field gel electrophoresis.

RESULTS:

430 samples were collected, and isolates of enterococci (n = 75) and staphylococci (110) were identified. Surfaces significantly associated with isolation of Enterococcus spp and Staphylococcus spp included cages and a weight scale. Fourteen Enterococcus spp isolates and 17 Staphylococcus spp isolates were resistant to ≥ 5 antimicrobials. Samples collected from the scale throughout the study suggested an overall increase in antimicrobial resistance of Enterococcus faecium over time. Clonality was detected for E faecium isolates collected from 2 different surfaces on the same day. CONCLUSIONS AND CLINICAL RELEVANCE Although not surprising, the apparent increase in antimicrobial resistance of E faecium was of concern because of the organism's ability to transmit antimicrobial resistance genes to other pathogens. Results reported here may aid in identification of critical control points to help prevent the spread of pathogens in VTHs.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staphylococcus / Enterococcus / Drug Resistance, Bacterial / Hospitals, Animal / Anti-Bacterial Agents Type of study: Observational_studies / Prevalence_studies / Risk_factors_studies Limits: Animals Language: En Journal: J Am Vet Med Assoc Year: 2012 Document type: Article Affiliation country: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staphylococcus / Enterococcus / Drug Resistance, Bacterial / Hospitals, Animal / Anti-Bacterial Agents Type of study: Observational_studies / Prevalence_studies / Risk_factors_studies Limits: Animals Language: En Journal: J Am Vet Med Assoc Year: 2012 Document type: Article Affiliation country: United States