Nanosphere lithography-based platform for developing rapid and high sensitivity microarray systems.
Biochem Biophys Res Commun
; 423(3): 473-7, 2012 Jul 06.
Article
in En
| MEDLINE
| ID: mdl-22683633
ABSTRACT
A novel gold nanoarray (NA)-based platform was developed for microarray applications. This novel approach is based upon the principle of nanosphere lithography and can be used for one-step antibody immobilization. The developed platform was checked by functionalizing with cysteine followed by capturing biotinylated antibody and detecting it with dye-conjugated steptravidin. An immunoassay was performed with spiked samples containing human fetuin A antigen. The minimum limits of detection (LOD) of human fetuin A for NA-based and conventional microarray platforms were 50 pg/mL and 50 ng/mL, respectively. The developed approach was highly reproducible and unlike conventional microarray approaches the use of a spotting system was omitted because immobilization was controlled and directed on the predefined arrays. This approach could be an ideal alternative for developing microarrays. And, the ease of the strategy also allows the high throughput production of the microarrays.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Printing
/
Microfluidic Analytical Techniques
/
Microarray Analysis
/
Metal Nanoparticles
/
Nanospheres
/
Gold
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2012
Document type:
Article
Affiliation country:
Ireland