An improved bioluminescence-based signaling assay for odor sensing with a yeast expressing a chimeric olfactory receptor.

Fukutani, Yosuke; Ishii, Jun; Noguchi, Keiichi; Kondo, Akihiko; Yohda, Masafumi

Fukutani, Yosuke; Ishii, Jun; Noguchi, Keiichi; Kondo, Akihiko; Yohda, Masafumi.

Affiliation

Fukutani Y; Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.

Biotechnol Bioeng ; 109(12): 3143-51, 2012 Dec.

Article in En | MEDLINE | ID: mdl-22729937

Subject(s)

Biotechnology/methods; Luminescent Measurements/methods; Odorants/analysis; Receptors, Odorant/metabolism; Saccharomyces cerevisiae/metabolism; Dinitrobenzenes/analysis; GTP-Binding Protein alpha Subunits/genetics; GTP-Binding Protein alpha Subunits/metabolism; Immunohistochemistry; Luciferases, Firefly/chemistry; Luciferases, Firefly/genetics; Luciferases, Firefly/metabolism; Promoter Regions, Genetic; Receptors, Odorant/genetics; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Signal Transduction

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Biotechnology / Receptors, Odorant / Luminescent Measurements / Odorants Language: En Journal: Biotechnol Bioeng Year: 2012 Document type: Article Affiliation country: Japan Country of publication: United States

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