Nitric oxide is involved in the regulation of trehalose accumulation under heat stress in Pleurotus eryngii var. tuoliensis.
Biotechnol Lett
; 34(10): 1915-9, 2012 Oct.
Article
in En
| MEDLINE
| ID: mdl-22763851
ABSTRACT
Little is known about the mechanism of how trehalose responds to various abiotic stresses although trehalose is considered as an important protectant in fungi. We investigated the role of nitric oxide (NO) in regulating trehalose accumulation during heat stress in Pleurotus eryngii var. tuoliensis. The addition of 100 or 200 g trehalose/l significantly inhibited the production of thiobarbituric acid-reactive substance under heat stress in mycelial cells. High temperature induced endogenous trehalose accumulation and sodium nitroprusside, a NO donor, further enhanced trehalose accumulation. Finally, heat-induced trehalose accumulation could be arrested by the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-1-oxyl-3-oxide, at 250 µM by inhibiting the transcription of trehalose phosphate synthase gene. Thus NO plays an important role in the regulation of trehalose accumulation during abiotic stresses in P. eryngii var. tuoliensis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trehalose
/
Heat-Shock Response
/
Pleurotus
/
Nitric Oxide
Language:
En
Journal:
Biotechnol Lett
Year:
2012
Document type:
Article
Affiliation country:
China