Characterizing the neurite outgrowth inhibitory effect of Mani.
FEBS Lett
; 586(19): 3018-23, 2012 Sep 21.
Article
in En
| MEDLINE
| ID: mdl-22771904
ABSTRACT
Mani (myelin-associated neurite-outgrowth inhibitor) protein is implicated in both axonal guidance and axonal regeneration after central nervous system (CNS) injury. Here, we applied a neurite outgrowth assay, coupled with a siRNA-driven investigation and immunocytochemistry, to unveil Mani's axonal outgrowth inhibitory effect in embryonic rat cortical primary neurons in vitro. We further demonstrate Mani's neuronal localization in comparison with a principal subunit, Cdc27, of the anaphase promoting complex (APC). Considering the protein structure of Mani obtained via a series of bio-computational studies, we propose a Cdc27-Mani-APC-related signalling pathway may be involved in CNS axon regeneration.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neurites
/
Nerve Tissue Proteins
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
2012
Document type:
Article
Affiliation country:
Singapore