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Determination of synthetic by-products and an intermediate in the colour additive D&C Orange No. 5 using high-performance liquid chromatography.
Yang, H-H W; Weisz, A.
Affiliation
  • Yang HH; Office of Cosmetics and Colors, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA.
Article in En | MEDLINE | ID: mdl-22779771
Specifications in the US Code of Federal Regulations for the colour additive D&C Orange No. 5 (Colour Index No. 45370:1) limit the levels of the synthetic by-products 2-(3,5-dibromo-2,4-dihydroxybenzoyl)benzoic acid (Br2BBA) and brominated resorcinol (Br3R) as well as the level of the intermediate phthalic acid (PhthAc). The present work reports the development and application of a high-performance liquid chromatography (HPLC) method for the quantitative determination of these impurities in D&C Orange No. 5 and its lakes. Br2BBA, Br3R and PhthAc were quantified by using five-point calibration curves with data points that ranged from 0.010% to 0.700%, from 0.012% to 0.706% and from 0.006% to 1.383% by weight, respectively. The HPLC method was applied to the analysis of test portions from 11 lots of D&C Orange No. 5 and one lot of D&C Orange No. 5 lake submitted to the US Food and Drug Administration (USFDA) for certification.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, High Pressure Liquid / Coloring Agents Language: En Journal: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Journal subject: CIENCIAS DA NUTRICAO Year: 2012 Document type: Article Affiliation country: United States Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, High Pressure Liquid / Coloring Agents Language: En Journal: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Journal subject: CIENCIAS DA NUTRICAO Year: 2012 Document type: Article Affiliation country: United States Country of publication: United kingdom