Expression of phosphoinositide-specific phospholipase C enzymes in human skin fibroblasts.
Connect Tissue Res
; 54(1): 1-4, 2013.
Article
in En
| MEDLINE
| ID: mdl-22800439
ABSTRACT
Fibroblasts are involved in a number of functions regulated by different signal transduction pathways, including the phosphoinositide (PI) signaling system and related converting enzymes, such as phosphoinositide-specific phospholipase C (PI-PLC). The PI-PLC family comprises crucial effector enzymes in the PI signal transduction pathway. Once activated, PI-PLC cleaves an important membrane PI, the phosphatidylinositol (4,5) bisphosphate into inositol trisphosphate and diacylglycerol-both are crucial molecules in the transduction of signals. The activity of selected PI-PLC enzymes was reported in fibroblasts, although the complete panel of expression was not available. Each cell type expresses a group of selected PI-PLC isoforms, and knowledge of the panel of expression is a necessary and preliminary tool to address further studies. In the present study, we delineated the expression panel of PI-PLC enzymes in human skin fibroblasts. PI-PLC ß1, PI-PLC ß3, PI-PLC ß4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, and PI-PLC ϵ were expressed. PI-PLC ß1 was weakly expressed, PI-PLC δ4 was inconstantly expressed, and PI-PLC γ2 was weakly expressed.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Gene Expression Regulation, Enzymologic
/
Phosphoinositide Phospholipase C
/
Fibroblasts
/
Isoenzymes
Limits:
Humans
Language:
En
Journal:
Connect Tissue Res
Year:
2013
Document type:
Article
Affiliation country:
Italy
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM