Antioxidant activity of propolis extracts from Serbia: a polarographic approach.
Food Chem Toxicol
; 50(10): 3614-8, 2012 Oct.
Article
in En
| MEDLINE
| ID: mdl-22842121
ABSTRACT
Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L(-1) alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L(-1) H2O2 decrease. Resulting HPEq ranged between 1.71±0.11 and 8.00±0.18 µL. Range of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093±0.004% to 0.346±0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31±0.05% g GAE, while the extract with the lowest activity contained 1.45±0.02% g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R2=0.991) and TCP (R2=0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polarography
/
Propolis
/
Antioxidants
Country/Region as subject:
Europa
Language:
En
Journal:
Food Chem Toxicol
Year:
2012
Document type:
Article