A convenient and general expression platform for the production of secreted proteins from human cells.

Aydin, Halil; Azimi, Farshad C; Cook, Jonathan D; Lee, Jeffrey E

Aydin, Halil; Azimi, Farshad C; Cook, Jonathan D; Lee, Jeffrey E.

Affiliation

Aydin H; Department of Laboratory Medicine and Pathobiology, University of Toronto.

J Vis Exp ; (65)2012 Jul 31.

Article in En | MEDLINE | ID: mdl-22872008

Subject(s)

Biotechnology/methods; Recombinant Proteins/biosynthesis; Cell Adhesion; Chromatography, Affinity; Culture Media, Conditioned; DNA/genetics; DNA/isolation & purification; HEK293 Cells; Humans; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Transfection/methods

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biotechnology / Recombinant Proteins Limits: Humans Language: En Journal: J Vis Exp Year: 2012 Document type: Article Country of publication: United States

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