Molecular characterization of oxysterol binding to the Epstein-Barr virus-induced gene 2 (GPR183).
J Biol Chem
; 287(42): 35470-35483, 2012 Oct 12.
Article
in En
| MEDLINE
| ID: mdl-22875855
ABSTRACT
Oxysterols are oxygenated cholesterol derivates that are emerging as a physiologically important group of molecules. Although they regulate a range of cellular processes, only few oxysterol-binding effector proteins have been identified, and the knowledge of their binding mode is limited. Recently, the family of G protein-coupled seven transmembrane-spanning receptors (7TM receptors) was added to this group. Specifically, the Epstein-Barr virus-induced gene 2 (EBI2 or GPR183) was shown to be activated by several oxysterols, most potently by 7α,25-dihydroxycholesterol (7α,25-OHC). Nothing is known about the binding mode, however. Using mutational analysis, we identify here four key residues for 7α,25-OHC binding Arg-87 in TM-II (position II20/2.60), Tyr-112 and Tyr-116 (positions III09/3.33 and III13/3.37) in TM-III, and Tyr-260 in TM-VI (position VI16/6.51). Substituting these residues with Ala and/or Phe results in a severe decrease in agonist binding and receptor activation. Docking simulations suggest that Tyr-116 interacts with the 3ß-OH group in the agonist, Tyr-260 with the 7α-OH group, and Arg-87, either directly or indirectly, with the 25-OH group, although nearby residues likely also contribute. In addition, Tyr-112 is involved in 7α,25-OHC binding but via hydrophobic interactions. Finally, we show that II20/2.60 constitutes an important residue for ligand binding in receptors carrying a positively charged residue at this position. This group is dominated by lipid- and nucleotide-activated receptors, here exemplified by the CysLTs, P2Y12, and P2Y14. In conclusion, we present the first molecular characterization of oxysterol binding to a 7TM receptor and identify position II20/2.60 as a generally important residue for ligand binding in certain 7TM receptors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Catalytic Domain
/
Receptors, G-Protein-Coupled
/
Molecular Docking Simulation
/
Hydroxycholesterols
Limits:
Humans
Language:
En
Journal:
J Biol Chem
Year:
2012
Document type:
Article
Affiliation country:
Denmark