An oleosin-fusion protein driven by the CaMV35S promoter is accumulated in Arabidopsis (Brassicaceae) seeds and correctly targeted to oil bodies.
Genet Mol Res
; 11(3): 2138-46, 2012 Aug 13.
Article
in En
| MEDLINE
| ID: mdl-22911597
ABSTRACT
Oleosin-fusion technology is used to express desired proteins. It was developed based on the properties of oleosin; the heterologous protein gene is fused to the oleosin gene and the fusion gene is driven by a seed-specific promoter. We replaced the seed specific promoter with the CaMV35S promoter to dive a gfp-oleosin fusion gene in transformed Arabidopsis. The heterologous oleosin-fusion protein was mainly accumulated in the transgenic Arabidopsis seeds and correctly targeted to oil bodies. This provides an alternate choice of promoter in oleosin-fusion technology.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Seeds
/
Recombinant Fusion Proteins
/
Organelles
/
Promoter Regions, Genetic
/
Arabidopsis
/
Caulimovirus
/
Arabidopsis Proteins
Language:
En
Journal:
Genet Mol Res
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2012
Document type:
Article
Affiliation country:
China