Optochemogenetics (OCG) allows more precise control of genetic engineering in mice with CreER regulators.
Bioconjug Chem
; 23(9): 1945-51, 2012 Sep 19.
Article
in En
| MEDLINE
| ID: mdl-22917215
ABSTRACT
New approaches that allow precise spatiotemporal control of gene expression in model organisms at the single cell level are necessary to better dissect the role of specific genes and cell populations in development, disease, and therapy. Here, we describe a new optochemogenetic switch (OCG switch) to control CreER/loxP-mediated recombination via photoactivatable ("caged") tamoxifen analogues in individual cells in cell culture, organoid culture, and in vivo in adult mice. This approach opens opportunities to more fully exploit existing CreER transgenic mouse strains to achieve more precise temporal- and location-specific regulation of genetic events and gene expression.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Engineering
Limits:
Animals
Language:
En
Journal:
Bioconjug Chem
Journal subject:
BIOQUIMICA
Year:
2012
Document type:
Article
Affiliation country:
United States