Cereal ß-glucan quantification with calcofluor-application to cell culture supernatants.
Carbohydr Polym
; 90(4): 1564-72, 2012 Nov 06.
Article
in En
| MEDLINE
| ID: mdl-22944417
ABSTRACT
The specific binding of the fluorescent dye calcofluor to cereal ß-glucan results in increased fluorescence intensity of the formed complex and is in use for the quantification of ß-glucan above a critical molecular weight (MW) by flow injection analysis. In this study, this method was applied in a fast and easy batch mode. In order to emphasize the spectral information of the emission spectra of the calcofluor/ß-glucan complexes, derivative signals were calculated. A linear relationship was found between the amplitude of the second derivative signals and the ß-glucan concentration between 0.1 and 0.4 µg/mL. The low detection limit of this new method (0.045 µg/mL) enabled its use to study the transport of cereal ß-glucans over differentiated Caco-2 cell monolayers. Additionally, the method was applied to quantify ß-glucan in arabinoxylan samples, which correlated well with data by an enzyme based method.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xylans
/
Benzenesulfonates
/
Edible Grain
/
Beta-Glucans
/
Fluorescent Dyes
Limits:
Humans
Language:
En
Journal:
Carbohydr Polym
Year:
2012
Document type:
Article
Affiliation country:
Norway