Analysis of the intraimplant microflora of two-piece dental implants.
Clin Oral Investig
; 17(4): 1135-42, 2013 May.
Article
in En
| MEDLINE
| ID: mdl-22961460
ABSTRACT
OBJECTIVE:
Information about the spectrum of microorganisms in the intraimplant cavities of two-piece dental implants is scarce. The purpose of this study was to assess the intraimplant microflora of two-piece dental implants by conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16 s rDNA gene sequencing. MATERIALS ANDMETHODS:
Ten patients (six men and four women; average age = 66.7 years; age range = 58-78 years) received 35 two-piece titanium implants carrying ball attachments. Biofilm sampling was performed with sterile microbrushes, and nonadherent microbial samples were obtained by injection and reuptake of predefined volumes of NaCl solution. The samples were cultured and analyzed by conventional biochemical testing, MALDI-TOF MS, and 16 s rDNA gene sequencing.RESULTS:
Of the 103 species detected, 27 and 33 were identified only in the biofilm and nonadherent microbial samples, respectively. Forty-three species were identified in both types of samples.CONCLUSIONS:
Two-piece dental implants harbored a broad spectrum of gram-positive and gram-negative aerobes and anaerobes, especially rods and cocci. CLINICAL RELEVANCE These findings confirm bacterial translocation from the oral cavity to intraimplant cavities. Microbiological methods as used in this study are necessary to reveal the complete vital microflora of intraimplant cavities.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dental Implants
/
Tooth Socket
/
Dental Implant-Abutment Design
/
Microbiota
Type of study:
Prognostic_studies
Limits:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Clin Oral Investig
Journal subject:
ODONTOLOGIA
Year:
2013
Document type:
Article
Affiliation country:
Germany