Selective RNase H cleavage of target RNAs from a tRNA scaffold.
Methods Mol Biol
; 941: 9-18, 2012.
Article
in En
| MEDLINE
| ID: mdl-23065550
ABSTRACT
In vivo overproduction of tRNA chimeras yields an RNA insert within a tRNA scaffold. For some applications, it may be necessary to discard the scaffold. Here we present a protocol for selective cleavage of the RNA of interest from the tRNA scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Transfer
/
RNA, Ribosomal, 16S
/
Ribonuclease H
/
RNA Cleavage
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2012
Document type:
Article
Affiliation country:
France