Skewed production of IL-6 and TGFß by cultured salivary gland epithelial cells from patients with Sjögren's syndrome.
PLoS One
; 7(10): e45689, 2012.
Article
in En
| MEDLINE
| ID: mdl-23118848
ABSTRACT
OBJECTIVE:
To determine the cytokine production profile of cultured salivary gland epithelial (SGE) cells obtained from patients with Sjögren's syndrome (SS).METHODS:
SGE cells obtained from 9 SS patients and 6 normal controls were cultured in the presence of exogenous IFNγ. Cell proliferation and apoptosis in response to IFNγ were determined by WST1 assay and by FACS analysis. The concentrations of IL-6 and TGFß secreted into culture supernatants were analyzed by ELISA.RESULTS:
IFNγ did not significantly affect the proliferation or apoptosis of SGE cells. However, IL-6 concentrations were higher, and TGFß concentrations were lower, in culture supernatants of SGE cells from SS patients than from normal controls.CONCLUSION:
Cytokine production by SGE cells from SS patients showed a skewed balance compared with normal controls, with increased IL-6 and decreased TGFß secretion. This imbalance may be critical in the regulation of Treg/Th17 cells and may foster a pathogenic milieu that may be causative and predictive in SS.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sjogren's Syndrome
/
Transforming Growth Factor beta
/
Interleukin-6
/
Epithelial Cells
Type of study:
Prognostic_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
PLoS One
Journal subject:
CIENCIA
/
MEDICINA
Year:
2012
Document type:
Article
Affiliation country:
Japan