Detection and sequencing of defective viral genomes in C6/36 cells persistently infected with dengue virus 2.
Arch Virol
; 158(3): 583-99, 2013 Mar.
Article
in En
| MEDLINE
| ID: mdl-23129130
ABSTRACT
Dengue virus is the most important arbovirus that affects humans, and it can establish persistent infections, especially in insect-derived cell cultures. Defective viral genomes have been implicated in the establishment and maintenance of persistent infections with several flaviviruses; however, there exists almost no information concerning defective dengue virus genomes. Here, we report the detection of defective dengue 2 virus genomes in persistently infected mosquito C6/36 cells. The defective viral genomes were detected at a low ratio compared with the wild-type genome. Deletions of approximately 147 residues (222-368) were found in the E protein, and these mainly affected domain III (73 %) of the protein; deletions of approximately 153 residues (4-156) and 228 residues (597-825) were found in the methyltransferase and polymerase domains, respectively, of the NS5 protein. The truncated versions of NS5 could be detected by western blot only in the protein extracts derived from persistently infected cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Envelope Proteins
/
Genome, Viral
/
Viral Nonstructural Proteins
/
Defective Viruses
/
Dengue Virus
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Arch Virol
Year:
2013
Document type:
Article
Affiliation country:
Mexico