Selection of test methods to be included in a testing strategy to predict acute oral toxicity: an approach based on statistical analysis of data collected in phase 1 of the ACuteTox project.
Toxicol In Vitro
; 27(4): 1377-94, 2013 Jun.
Article
in En
| MEDLINE
| ID: mdl-23178337
ABSTRACT
More than 50 different in vitro and in silico methods assessing specific organ- and system-toxicity, such as haemato-, neuro-, nephro- and hepatotoxicity, as well as intestinal absorption, distribution and metabolism, have been used in the first phase of the ACuteTox project to test a common set of 57 chemicals. This paper describes the methods used for statistical evaluation of concentration-response data collected for each of the endpoint assays, and for the development of a testing strategy applicable for acute toxicity classification of chemicals based on the achieved results of the concentration-response analysis. A final list of in vitro test methods considered to be promising candidates for building blocks of the testing strategy is presented. Only these selected test methods were further investigated in the prevalidation phase of the project. The test methods were chosen according to their reproducibility and reliability and most importantly, according to their potential to classify chemicals into the official acute oral toxicity categories of the EU Classification, Labelling and Packaging (CLP) Regulation. The potential of the test methods to correctly classify the chemicals was assessed by Classification and Regression Trees (CART) analysis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Toxicity Tests, Acute
Type of study:
Etiology_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Toxicol In Vitro
Journal subject:
TOXICOLOGIA
Year:
2013
Document type:
Article
Affiliation country:
Italy