Characterisation of AmphiAmR4, an amphioxus (Branchiostoma floridae) α2-adrenergic-like G-protein-coupled receptor.
Invert Neurosci
; 13(1): 71-84, 2013 Jun.
Article
in En
| MEDLINE
| ID: mdl-23183848
ABSTRACT
Little is known about the evolutionary relationship between vertebrate adrenergic receptors and invertebrate octopamine and tyramine receptors. The complexity of the adrenergic signalling system is believed to be an innovation of the vertebrate lineage but the presence of noradrenaline has been reported in some invertebrate species. The cephalochordate, amphioxus (Branchiostoma floridae), is an ideal model organism for studying the evolution of vertebrate GPCRs, given its unique position at the base of the chordate lineage. Here, we describe the pharmacological characterisation and second messenger coupling abilities of AmphiAmR4, which clusters with α2-adrenergic receptors in a phylogenetic tree but also shares a high sequence similarity to invertebrate octopamine/tyramine receptors in both BLAST and Hidden Markov Model analyses. Thus, it was of particular interest to determine if AmphiAmR4 displayed similar functional properties to the vertebrate α2-adrenergic receptors or to invertebrate octopamine or tyramine receptors. When stably expressed in Chinese hamster ovary (CHO) cells, noradrenaline couples the receptor to both the activation of adenylyl cyclase and to the activation of the MAPKinase pathway. Pharmacological studies with a wide range of agonists and antagonists suggest that AmphiAmR4 functions as an α2-adrenergic-like receptor when expressed in CHO cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Adrenergic, alpha
/
Receptors, G-Protein-Coupled
/
Lancelets
Limits:
Animals
Language:
En
Journal:
Invert Neurosci
Journal subject:
NEUROLOGIA
Year:
2013
Document type:
Article
Affiliation country:
United kingdom