Fungal pretreatment of switchgrass for improved saccharification and simultaneous enzyme production.
Bioresour Technol
; 135: 39-45, 2013 May.
Article
in En
| MEDLINE
| ID: mdl-23195655
ABSTRACT
This study investigates fungal pretreatment of switchgrass involving solid state fermentation (SSF) to improve saccharification and simultaneously produce enzymes as co-products. The results revealed that the fungus Pycnoporus sp. SYBC-L3 can significantly degrade lignin and enhance enzymatic hydrolysis efficiency. After a 36-d cultivation period, nearly 30% reduction in lignin content was obtained without significant loss of cellulose and hemicellulose, while a considerable amount of laccase, as high as 6.3 U/g, was produced. After pretreatment, pores on switchgrass surface were observed using scanning electron microscopy (SEM). The enzymatic hydrolysis efficiency for the switchgrass with 36-d pretreatment was about 50% greater than the untreated one. Our results suggest that solid state fungal cultivation may be a good method for switchgrass pretreatment, which can simultaneously achieve high efficiency of enzymatic hydrolysis and production of some useful enzymes for other industrial utilization.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biotechnology
/
Enzymes
/
Carbohydrate Metabolism
/
Pycnoporus
/
Poaceae
Language:
En
Journal:
Bioresour Technol
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2013
Document type:
Article
Affiliation country:
United States