Multiplex protein detection with DNA readout via mass spectrometry.
N Biotechnol
; 30(2): 153-8, 2013 Jan 25.
Article
in En
| MEDLINE
| ID: mdl-23201185
ABSTRACT
Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Proteins
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
N Biotechnol
Journal subject:
BIOLOGIA MOLECULAR
/
ENGENHARIA BIOMEDICA
Year:
2013
Document type:
Article
Affiliation country:
United States