[Apoptotic mechanism of gecko crude peptides on human liver carcinoma cell line HepG2].
Zhong Yao Cai
; 35(6): 863-6, 2012 Jun.
Article
in Zh
| MEDLINE
| ID: mdl-23236815
ABSTRACT
OBJECTIVE:
To investigate the effect of Gecko crude peptides (GCPS) on human liver carcinoma HepG2 cells and its mechanism.METHODS:
MTT assay was used to analyze the effect of the GCPS on the proliferation of HepG2 Cell; Nucleus change of HepG2 treated with GCP was observed by Hoechst33258 fluorescence staining, and BAX and BCL-2 were detected with western-blot assay.RESULTS:
GCPS could inhibit the proliferation of HepG2 Cell in a time and dosage dependent way, and its half-maximal inhibitory concentration (IC50) was 1.2 mg/mL; HepG2 pretreated with GCPS showed apoptotic morphological changes. GCPS (1.6 mg/mL, 0.8 mg/mL) could decrease the expression of BCL-2 protein, and increase the expression of BAX protein.CONCLUSION:
GCPS can inhibit the proliferation of HepG2 cell. The mechanism may be related to the induction apoptosis of HepG2.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Materia Medica
/
Apoptosis
/
Cell Proliferation
/
Lizards
/
Antineoplastic Agents
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Zhong Yao Cai
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2012
Document type:
Article
Affiliation country:
China
Publication country:
CHINA
/
CN
/
REPUBLIC OF CHINA