Synaptic vesicle protein 2 (SV2) isoforms.
Asian Pac J Cancer Prev
; 13(10): 5063-7, 2012.
Article
in En
| MEDLINE
| ID: mdl-23244111
ABSTRACT
New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics, including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. The purpose of this research was to determine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, B and C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their protein and mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation to neural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2 as molecular marker and a vehicle for cancer treatment with botulinum toxin.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breast
/
Breast Neoplasms
/
Membrane Glycoproteins
/
Nerve Tissue Proteins
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
En
Journal:
Asian Pac J Cancer Prev
Journal subject:
NEOPLASIAS
Year:
2012
Document type:
Article
Affiliation country:
Mexico