Molecular methods of virus detection in lymphoma.
Methods Mol Biol
; 971: 277-93, 2013.
Article
in En
| MEDLINE
| ID: mdl-23296970
ABSTRACT
The herpesviruses Epstein-Barr virus (EBV) and human herpesvirus 8 and the retrovirus human T-cell leukemia virus type 1 are directly implicated in the pathogenesis of lymphoma and leukemia in man. EBV is associated with an expanding spectrum of lymphomas and it would appear likely that additional, possibly novel, viruses will be implicated in lymphoma pathogenesis in the future. This chapter describes techniques that may be useful in the analysis of viruses and lymphoma including a standard EBV EBER in situ hybridization assay and a degenerate PCR assay for detection of novel herpesviruses. Lastly, a method for analysis of next-generation sequences in the quest for novel viruses is described.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
In Situ Hybridization
/
Herpesvirus 4, Human
/
High-Throughput Nucleotide Sequencing
/
Lymphoma
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2013
Document type:
Article
Affiliation country:
United kingdom