The role of aldehyde/alcohol dehydrogenase (AdhE) in ethanol production from glycerol by Klebsiella pneumoniae.
J Ind Microbiol Biotechnol
; 40(2): 227-33, 2013 Feb.
Article
in En
| MEDLINE
| ID: mdl-23296976
ABSTRACT
Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5 g l(-1), when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9 g l(-1) under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Alcohol Dehydrogenase
/
Ethanol
/
Aldehyde Dehydrogenase
/
Glycerol
/
Klebsiella pneumoniae
Language:
En
Journal:
J Ind Microbiol Biotechnol
Journal subject:
BIOTECNOLOGIA
/
MICROBIOLOGIA
Year:
2013
Document type:
Article