Skin lipid profiling in normal and seborrhoeic shih tzu dogs.
Vet Dermatol
; 24(1): 84-9.e21-2, 2013 Feb.
Article
in En
| MEDLINE
| ID: mdl-23331684
ABSTRACT
BACKGROUND:
Seborrhoea is a clinical condition resulting in excessive lipid and/or scale on the skin and is a common and important skin disease of dogs. However, there is little information on the skin surface lipid composition of dogs with seborrhoea. HYPOTHESIS/OBJECTIVES:
To compare skin surface lipid profiles in normal and seborrhoeic shih tzu dogs.METHODS:
Fourteen client-owned dogs (seven seborrhoeic and seven normal) were investigated. Lipids in sebaceous glands (SGs) were extracted from homogenized tissues of SG hyperplasia. Surface lipid was collected by tape stripping [stratum corneum (SC)-enriched fraction] and acetone-wetted cotton swab (acetone-extracted fraction). Lipids in SGs, SC-enriched fractions and acetone-extracted fractions were evaluated by high-performance thin-layer chromatography.RESULTS:
Lipids in SGs mainly consisted of cholesterol esters, wax esters and triglycerides, whereas lipids in the SC-enriched fraction mainly consisted of ceramides. The acetone-extracted fraction contained a mixture of lipid classes recognized in SG- and SC-enriched fractions. In seborrhoeic dogs, concentrations of wax esters and triglycerides in the acetone-extracted fraction were significantly higher than in control dogs (P = 0.0285). Amounts of total ceramides (in micrograms) per milligram of SC were not significantly different between the two groups (P = 0.5204). Interestingly, two unknown ceramide fractions, which accounted for 20% of the total ceramides, were recognized exclusively in seborrhoeic dogs. CONCLUSIONS AND CLINICAL IMPORTANCE These results provide evidence that the skin surface lipid profiles are altered in shih tzu dogs with seborrhoea.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Dermatitis, Seborrheic
/
Dog Diseases
/
Lipid Metabolism
Type of study:
Observational_studies
Limits:
Animals
Language:
En
Journal:
Vet Dermatol
Journal subject:
DERMATOLOGIA
/
MEDICINA VETERINARIA
Year:
2013
Document type:
Article
Affiliation country:
Japan