Ceramide 1-phosphate stimulates glucose uptake in macrophages.
Cell Signal
; 25(4): 786-95, 2013 Apr.
Article
in En
| MEDLINE
| ID: mdl-23333242
ABSTRACT
It is well established that ceramide 1-phosphate (C1P) is mitogenic and antiapoptotic, and that it is implicated in the regulation of macrophage migration. These activities require high energy levels to be available in cells. Macrophages obtain most of their energy from glucose. In this work, we demonstrate that C1P enhances glucose uptake in RAW264.7 macrophages. The major glucose transporter involved in this action was found to be GLUT 3, as determined by measuring its translocation from the cytosol to the plasma membrane. C1P-stimulated glucose uptake was blocked by selective inhibitors of phosphatidylinositol 3-kinase (PI3K) or Akt, also known as protein kinase B (PKB), and by specific siRNAs to silence the genes encoding for these kinases. C1P-stimulated glucose uptake was also inhibited by pertussis toxin (PTX) and by the siRNA that inhibited GLUT 3 expression. C1P increased the affinity of the glucose transporter for its substrate, and enhanced glucose metabolism to produce ATP. The latter action was also inhibited by PI3K- and Akt-selective inhibitors, PTX, or by specific siRNAs to inhibit GLUT 3 expression.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ceramides
/
Glucose
/
Macrophages
Limits:
Animals
Language:
En
Journal:
Cell Signal
Year:
2013
Document type:
Article
Affiliation country:
Spain